Thursday, September 12, 2013

10:00 – 18:00   Registration and poster mounting

11:00 – 13:00   Guided tour to Sümeg Castle with lunch (optional)

17:00                 Introductory Remarks

                           Plenary Lecture

17:15                 Michal Toborek

               (University of Miami, USA)

               Targeting the gut-brain axis for modulation of brain  metastasis formation

                           NEW DEVELOPMENTS

                                  Chair: Winfried Neuhaus

18:00                 N Joan Abbott
                           (King’s College London, UK)
                           An in vitro ‘double-decker’ blood-brain barrier (DD-BBB) co-culture
                           model: combining permeability assay and functional assessment
                           of glioblastoma treatment

18:20                 Marie-Pierre Dehouck
(University Lille Nord de France, U. Artois, France)
                           A stable and reproducible human blood-brain barrier model derived from stem cells

18:40                 Pierre-Olivier Couraud
                           (Institut Cochin, Paris, France)

               Pharmacological studies and pathogen interactions
               with human brain endothelial cells in vitro
               under flow

19:00                 Welcome Reception          


Friday, September 13, 2013     

                         OF BBB PERMEABILITY

                         Chairs: Ingolf Blasig, Walter Hunziker


9:00                  Anuska Andjelkovic
University of Michigan, Ann Arbor, USA)
    Cortactine-ZO-1 interaction regulates stability of tight junctional complex


9:20                  Walter Hunziker
(Institute of Molecular and Cell Biology, Singapore)
    Physiological roles of zonula occludens proteins


9:40                  Tetsuya Terasaki
    (Tohoku University, Sendai, Japan)

    Expression and function of claudin-11 in human  blood-brain barrier


10:00               Ingolf Blasig
    (Leibniz-Institute of Molecular Pharmacology, Berlin, Germany)
    Binding measurements of claudin ligands by means  of microscale thermophoresis


10:20               Coffee break


11:00               Jimmi Cording
(Leibniz-Institute of Molecular Pharmacology, Berlin, Germany)

    Tricellulin is sensitive to pathological conditions    such as hypoxia and
    related conditions


11:20                Anja Feldner
(German Cancer Research Center, Heidelberg, Germany)

    MPDZ controls blood-brain-barrier function


11:40                Daniela Virgintino
(University of Bari, Italy)

              CXCL12 secretion by radial glia (rg) cells is involved in glio-vascular
              communication during human brain vascularization

12:00                Lunch


                          BBB AND DRUG DELIVERY TO THE BRAIN

                          Chairs: David Begley, Tetsuya Terasaki                      

 13:40               Birger Brodin
     (University of Copenhagen, Denmark)
     The brain-to-blood transport of the excitotoxic amino acid
     L-glutamate by the endothelial cells of the blood-brain barrier is
     mediated by the concerted action of abluminal excitatory amino
     acid transporter 1 and a luminal, L-glutamate carrier


14:00                David Male
(The Open University, UK)

     Delivery of glucose-coated gold nanoparticles to astrocytes by transfer
     across human brain endothelium


14:20                Svetlana Drndarski
(King’s College London, UK)

     Neurotrophins and the BBB: some new evidence of  crossing


14:40                Brankatschk Marko
(Max Planck Institute for Molecular Cell Biology and Genetics Dresden, Germany)

                          Lipoprotein particles cross the BBB and regulate insulin signaling in drosophila


15:00                Christian Staat
     (Leibniz-Institute of Molecular Pharmacology, Berlin, Germany)

                          C1C2, a claudin-derived peptide to enhance drug delivery - mode of action

15:20                Lajos Szente
(CycloLab, Budapest, Hungary)

     Cyclodextrins as active pharmacutical agents


15:40                Coffee Break


16:00 – 18:00  Poster session I

                          P1 Sonia Al-qadi (University of Copenhagen, Denmark)
                          An insect-based in vivo/ex vivo model for drug permeability
                          screening across the blood brain barrier

                          P2 Annette Burkhart (Aalborg University, Denmark)
Transfection of rat brain endothelium in a primary culture
                          model of the blood-brain barrier at different states of barrier maturity

                          P3 Louiza B. Thomsen (Aalborg University, Denmark)
                          Characterisation of an in vitro blood-brain barrier model based
                          on primary porcine capillary endothelial cells in monoculture or co-culture
                          with primary rat or porcine astrocytes and pericytes

                          P4 Christian Tscheik (Leibniz-Institute of Molecular Pharmacology, Berlin,Germany)
                          Different cell culture models to investigate tight junction proteins

                          P5 Daniela E. Eigenmann (University of Basel, Switzerland)
                          Comparative study of four immortalized human brain capillary
                          endothelial cell lines, hCMEC/D3, hBMEC, TY10 and BB19 and optimization
                          of culture conditions, for an in vitro blood-brain barrier model for
                          drug permeability studies

                          P6 András Kincses (Biological Research Centre, HAS, Szeged, Hungary)
                          Lab-on-a-chip tool for modelling biological barriers

                          P7 Antje Appelt-Menzel (University Clinic Würzburg, Germany)
                          Development of a 3D in vitro model of the blood brain barrier

                          P8 Andrea Harrer (Paracelsus Medical University, Salzburg, Austria)
                          Is CSF immunologically active? An in vitro approach to inside-out
                          signaling via brain microvascular endothelial cells

                          P9 Nora Gehne (Leibniz-Institute of Molecular Pharmacology, Berlin, Germany)
                          mTurquoise2: a new CFP-variant for studying interactions between
                          tight junction proteins of the blood-brain barrier

                          P10 Stefanie E. Herberich (German Cancer Research Center, Heidelberg, Germany)
                          NOTCH signaling is critical for endothelial barrier functions

                          P11 Petra Hülper (University of Göttingen, Germany)
                          Influence of short-chain alkylglycerols and hypertonic mannitol on
                         primary brain endothelial cells

                         P12 Alexandra Bocsik (Biological Research Centre, HAS, Szeged, Hungary)
                         Peptides acting on intercellular junctions reversibly open biological barriers

                         P13 Lars Winkler (Leibniz-Institute of Molecular Pharmacology, Berlin, Germany)
                         Quantification of tight junction protein expression in brain capillary endothelial cells

                         P14 Nemanja Milicevic (Leibniz-Institute of Molecular Pharmacology, Berlin, Germany)
                         Investigation of biomolecular interactions with the help of microscale thermophoresis

                         P15 Mareen Lüthen (Free University Berlin, Germany)
                         Physiological effects of occludin outside the tight junction environment

                         P16 Rosel Blasig (Leibniz-Institute of Molecular Pharmacology, Berlin, Germany)
                         Colocalization of tight junction proteins from murine brain capillaries
                         with ZO-1 under normal and hypoxic conditions

                         P17 Shalmali Patkar (University of Zurich, Switzerland)
                         Crosstalk between hypoxia inducible factor-1 and Rho-GTPase
                         signaling: hypoxia-induced cytoskeletal re-arrangements at the BBB.

                         P18 Victoria Makrides (University of Zurich, Switzerland)
                         Blood-brain barrier amino acid transporter regulation of brain
                         interstitial glutamine concentrations – an in vitro study

                         P19 Elena Dolgodilina (University of Zurich, Switzerland)
                         The role of BBB amino acid transporters in brain interstitial fluid
                         amino acid homeostasis probed by in vivo brain microdialysisin
                         awake and freely moving mice

                         P20 Steffen Strock (University Medical Center Mainz, Germany)
                         The role of LRP1 in Aβ clearance across the blood-brain barrier in Alzheimer’s disease

                         P21 Reiner F. Haseloff (Leibniz-Institute of Molecular Pharmacology, Berlin, Germany)
                         Identification of potential biomarkers for evaluation of neurodegenerative
                         disorders – perspectives and pitfalls

                         P22 Hans-Christian Bauer (Paracelsus Medical University, Salzburg, Austria)
                         Protein transport mechanism at the developing mouse choroid
                         plexus: a molecular characterization

                         P23 Aleksandra Szczepkowska (Institute of Animal Reproduction and
                         Food Research, PAS, Olstyn, Poland)

                         Melatonin implantation during long days up-regulates mRNA expression
                         of aquaporins (AQP1 and AQP4) and claudin-2 in the ovine choroid plexus

                         P24 Janina Skipor (Institute of Animal Reproduction and Food Research,
                         PAS, Olstyn, Poland)

                         oes melatonin affect mRNA expression of toll-like receptors in the ovine choroid plexus?

                         P25 Ádám Nyúl-Tóth (Biological Research Centre, HAS, Szeged, Hungary)
                         Toll-like receptors in brain endothelial cells: expression and role in barrier regulation

                         P26 Imola Wilhelm (Biological Research Centre, HAS, Szeged, Hungary)
                         Role of PACAP in the regulation of survival and barrier properties of cerebral
                         endothelial cells


19:00               Wine tasting and dinner


 Saturday, September 14, 2013


N Joan Abbott, Alon Friedman


9:00                    Alon Friedman
(Ben-Gurion University of the Negev, Israel)

      Measuring brain vascular permeability in clinical
      practice: why, when and how


9:20                    Shilpa Buch
(University of Nebraska, Omaha, USA)

      HIV Tat-mediated induction of platelet-derived 
      growth factor in pericytes: implications for pericyte migration


9:40                    Ibolya András
(University of Miami, USA)
      HIV-1 induced nuclear amyloid beta uptake is
      regulated by the Smad signaling pathway in brain endothelial cells


10:00                  Victor Castro
(University of Miami, USA)

                Occludin controls metabolic integrative pathways   and modulates HIV-infection

10:20                  Coffee break


11:00                  Fabien Gosselet
(University Lille Nord de France,
U. Artois, France)
Oxysterols, liver X receptors (Lxrs) & Alzheimer’s
disease: focus at the blood-brain barrier (BBB) level


11:20                   Claus Pietrzik
(Institute of Pathobiochemistry, University Medical Center Mainz, Germany)

       Nanoparticulate flurbiprofen affects Aβ42    generation in an in vitro blood-brain
       barrier model


11:40                   Sandra Orozco Suárez
       (Instituto Mexicano del Seguro Social, Mexico)

       Structural alterations in brain barrier components in

       Rasmussen encephalitis

12:00                   Lunch



                 Chairs: Shilpa Buch, Roméo Cecchelli



13:40                  Ágnes Kittel
                (Institute of Experimental Medicine, HAS, Budapest, Hungary)

                Ultrastructural changes of blood-brain barrier in l-   ornithine induced pancreatitis


14:00                  Fruzsina Walter
(Biological Research Centre, HAS, Szeged, Hungary)

      Effects of l-ornithine on cultured primary brain endothelial cells


14:20                  Colin Willis
      (University of New England, Biddeford, USA
Integrin receptor mediated oxidative stress modulates blood-brain barrier integrity


14:40                  Zoran Redzic
      (Kuwait University, Kuwait)
      Differential effects of paracrine factors on the   survival of cells
      of the neurovascular unit during oxygen glucose deprivation


15:00                  Sabrina Engelhardt  
       (University of Zurich, Switzerland)
      The BBB under hypoxic attack – a comparative study on cell-specific responses


15:20                  Coffee break


15:40 – 17:20    Poster session II



P27 Csilla Fazakas (Biological Research Centre, HAS, Szeged, Hungary)
Role of rho-kinases (ROCK) in melanoma brain metastasis formation


P28 Judit Molnár (Biological Research Centre, HAS, Hungary)

Signal transduction pathways involved in the transmigration of melanoma
cells through the blood-brain barrier


P29 János Haskó (Biological Research Centre, HAS, Hungary)
Role of cannabinoid receptor 2 in the formation of brain metastases of melanoma


P30 Francisca Fernandez-Valverde (Instituto Nacional de Neurología y
Neurocirugía, Mexico City, Mexico)

Ultrastructural characteristics of blood–brain barrier in cerebral lymphoma metastases


P31 Mehmet Kaya (Istanbul University, Turkey)

Exogenous beta hydroxybutyrate alters blood-brain barrier integrity in normal rats


P32 Anne Winkler (University of Göttingen, Germany)

Early breakdown of the blood-brain barrier in a model of neuromyelitis optica


P33 András Harazin (Biological Research Centre, HAS, Szeged, Hungary)

Blood-brain barrier opening and gene expression of cationic amino
acid transporter-1 in brain microvessels in l-ornithine induced acute pancreatitis in rats


P34 Andrea E. Tóth (Biological Research Centre, HAS, Szeged, Hungary)

Edaravone protects against acute carbonyl stress in human brain endothelial cells

P35 Winfried Neuhaus (University of Vienna, Austria)

Novel therapeutical in-vitro approaches for stroke treatment: The role of the
tPA/PAI-1 axis at the blood-brain barrier

P36 Masahiro Nishibori(Okayama University, Japan)

Protection of BBB disruption in ischemic and traumatic injuries by
anti-HMGB1 monoclonal antibody

P37 Shuji Fukuda (Nagasaki University, Japan)

Cilostazol improves the BBB barrier functions against AGEs-related ischemic damage via TGF-β signaling


P38 Yoichi Morofuji (Nagasaki University, Japan)

Pitavastatin ameliorates lipopolysaccharide-induced dysfunction of blood-brain barrier

P39 Dong Hyun Jo (Seoul National University, Republic of Korea)

Therapeutic potential of metal nanoparticles on pathologic angiogenesis in the retina

P40 Sabrina Meister (University Medical Center Mainz, Germany)

         Impaired BBB integrity of ALS spinal cord derived endothelial cells

P41 Péter Sipos (University of Szeged, Hungary)

Physicochemical characterization of nanosized vesicular carriers for drug delivery to brain

P42 Lóránd Kiss (Biological Research Centre, HAS, Szeged, Hungary)

Nanovesicles for drug delivery across blood-brain barrier: a cell culture study


P43 Ildikó Bácskay (University of Debrecen, Hungary)

Evaluation of the relations between structure and cytotoxic effects and
paracellular transport enhancer effect of different α-cyclodextrin derivatives


P44 Piotr Ruszkowski (Poznan University, Poland)

In vitro studies on lamotrigine blood-brain barrier permeability and
its anticancer activity against glioma cancer cells


P45 Sofia Gustafsson (Uppsala University, Sweden)

Combined microPET imaging and microdialysis sampling in a study
of oxycodone active uptake in rat brain


P46 Annika Lindqvist (Uppsala University, Sweden)

Mechanistic evaluation of the increaised brain delivery of the opioid peptide


P47 Lorena Reyes Santos (Instituto Nacional de Neurología y Neurocirugía, Mexico)

Effect of systemic tuberculosis in the neuronal and blood-brain barrier alterations in a murine experimental model.


P48 Petra Sántha (Biological Research Centre, HAS, Szeged, Hungary)

Stress induced alterations in the morphology of the neurovascular unit


P49 Francisca Tristan Agundis (Hospital Psiquiátrico Fray Bernardino
Álvarez, Mexico City, Mexico)

Morphological impairment of blood-brain barrier in Alzheimer’s disease


P50 Inês Sá-Pereira (University of Oxford, UK)
Neurodevelopmental response to intracerebral inflammation

P51 Juana Villeda-Hernández (Hospital de especialidades del Centro
Médico Nacional, Mexico City, Mexico)

Structural and ultrastructural characteristics in blood brain barrier in
neocortex of patients with refractory epilepsy


P52 Udi Vazana (Ben-Gurion Univeristy of the Negev, Israel)

Low-frequency transcranial magnetic stimulation enhances blood-brain barrier permeability: a combined animal and human study


P53 Colin Willis (University of New England, Biddeford, USA)

Migraine medication induces AKT phosphorylation and modulates blood-brain
barrier integrity


P54 Andrej Kovac (Institute of Neuroimmunology, Slovak Academy of Sciences, Slovakia)

OspA-CD40 interaction is important for translocation of neuroinvasive Borrelia across the blood-brain barrier



18:00                  Historical Tournaments and Medieval Dinner




Sunday, September 15, 2013



Breakfast and check out


Dismounting posters


Shuttle buses to Liszt Ferenc International Airport, Budapest