Programme

9:00–12:00    SESSION II.: MOLECULAR MECHANISMS OF THE REGULATION
                         OF BBB PERMEABILITY

                         Chairs: Ingolf Blasig, Walter Hunziker

9:00                  Anuska Andjelkovic
    (University of Michigan, Ann Arbor, USA)
    Cortactine-ZO-1 interaction regulates stability of tight junctional complex

9:20                  Walter Hunziker
    (Institute of Molecular and Cell Biology, Singapore) 
    Physiological roles of zonula occludens proteins

9:40                  Tetsuya Terasaki
    (Tohoku University, Sendai, Japan)
    Expression and function of claudin-11 in human  blood-brain barrier

10:00               Ingolf Blasig
    (Leibniz-Institute of Molecular Pharmacology, Berlin, Germany) 
    Binding measurements of claudin ligands by means  of microscale thermophoresis

10:20               Coffee break

11:00               Jimmi Cording
    (Leibniz-Institute of Molecular Pharmacology, Berlin, Germany)
    Tricellulin is sensitive to pathological conditions    such as hypoxia and Ű
    related conditions

11:20                Anja Feldner
    (German Cancer Research Center, Heidelberg, Germany)
    MPDZ controls blood-brain-barrier function

11:40                Daniela Virgintino
    (University of Bari, Italy)

              CXCL12 secretion by radial glia (rg) cells is involved in glio-vascular
              communication during human brain vascularization

12:00                Lunch

13:40–15:40  SESSION III.: TRANSPORT PROCESSES OF THE
                          BBB AND DRUG DELIVERY TO THE BRAIN
                          Chairs: David Begley, Tetsuya Terasaki                      

 13:40               Birger Brodin
     (University of Copenhagen, Denmark)
     The brain-to-blood transport of the excitotoxic amino acid
     L-glutamate by the endothelial cells of the blood-brain barrier is
     mediated by the concerted action of abluminal excitatory amino
     acid transporter 1 and a luminal, L-glutamate carrier

14:00                David Male
     (The Open University, UK)

     Delivery of glucose-coated gold nanoparticles to astrocytes by transfer
     across human brain endothelium

14:20                Svetlana Drndarski
     (King’s College London, UK)
     Neurotrophins and the BBB: some new evidence of  crossing

14:40                Brankatschk Marko
     (Max Planck Institute for Molecular Cell Biology and Genetics Dresden, Germany)

                          Lipoprotein particles cross the BBB and regulate insulin signaling in drosophila

15:00                Christian Staat
     (Leibniz-Institute of Molecular Pharmacology, Berlin, Germany)

                          C1C2, a claudin-derived peptide to enhance drug delivery - mode of action

15:20                Lajos Szente
     (CycloLab, Budapest, Hungary)
     Cyclodextrins as active pharmacutical agents

15:40                Coffee Break

16:00 – 18:00  Poster session I

                          P1 Sonia Al-qadi (University of Copenhagen, Denmark)
                          An insect-based in vivo/ex vivo model for drug permeability
                          screening across the blood brain barrier

                          P2 Annette Burkhart (Aalborg University, Denmark)
                          Transfection of rat brain endothelium in a primary culture
                          model of the blood-brain barrier at different states of barrier maturity

                          P3 Louiza B. Thomsen (Aalborg University, Denmark)
                          Characterisation of an in vitro blood-brain barrier model based
                          on primary porcine capillary endothelial cells in monoculture or co-culture
                          with primary rat or porcine astrocytes and pericytes

                          P4 Christian Tscheik (Leibniz-Institute of Molecular Pharmacology, Berlin,Germany)
                          Different cell culture models to investigate tight junction proteins

                          P5 Daniela E. Eigenmann (University of Basel, Switzerland)
                          Comparative study of four immortalized human brain capillary
                          endothelial cell lines, hCMEC/D3, hBMEC, TY10 and BB19 and optimization
                          of culture conditions, for an in vitro blood-brain barrier model for
                          drug permeability studies

                          P6 András Kincses (Biological Research Centre, HAS, Szeged, Hungary)
                          Lab-on-a-chip tool for modelling biological barriers

                          P7 Antje Appelt-Menzel (University Clinic Würzburg, Germany)
                          Development of a 3D in vitro model of the blood brain barrier

                          P8 Andrea Harrer (Paracelsus Medical University, Salzburg, Austria)
                          Is CSF immunologically active? An in vitro approach to inside-out
                          signaling via brain microvascular endothelial cells

                          P9 Nora Gehne (Leibniz-Institute of Molecular Pharmacology, Berlin, Germany)
                          mTurquoise2: a new CFP-variant for studying interactions between
                          tight junction proteins of the blood-brain barrier

                          P10 Stefanie E. Herberich (German Cancer Research Center, Heidelberg, Germany)
                          NOTCH signaling is critical for endothelial barrier functions

                          P11 Petra Hülper (University of Göttingen, Germany)
                          Influence of short-chain alkylglycerols and hypertonic mannitol on
                         primary brain endothelial cells

                         P12 Alexandra Bocsik (Biological Research Centre, HAS, Szeged, Hungary)
                         Peptides acting on intercellular junctions reversibly open biological barriers

                         P13 Lars Winkler (Leibniz-Institute of Molecular Pharmacology, Berlin, Germany)
                         Quantification of tight junction protein expression in brain capillary endothelial cells

                         P14 Nemanja Milicevic (Leibniz-Institute of Molecular Pharmacology, Berlin, Germany)
                         Investigation of biomolecular interactions with the help of microscale thermophoresis

                         P15 Mareen Lüthen (Free University Berlin, Germany)
                         Physiological effects of occludin outside the tight junction environment

                         P16 Rosel Blasig (Leibniz-Institute of Molecular Pharmacology, Berlin, Germany)
                         Colocalization of tight junction proteins from murine brain capillaries
                         with ZO-1 under normal and hypoxic conditions

                         P17 Shalmali Patkar (University of Zurich, Switzerland)
                         Crosstalk between hypoxia inducible factor-1 and Rho-GTPase
                         signaling: hypoxia-induced cytoskeletal re-arrangements at the BBB.

                         P18 Victoria Makrides (University of Zurich, Switzerland)
                         Blood-brain barrier amino acid transporter regulation of brain
                         interstitial glutamine concentrations – an in vitro study

                         P19 Elena Dolgodilina (University of Zurich, Switzerland)
                         The role of BBB amino acid transporters in brain interstitial fluid
                         amino acid homeostasis probed by in vivo brain microdialysisin
                         awake and freely moving mice

                         P20 Steffen Strock (University Medical Center Mainz, Germany)
                         The role of LRP1 in Aβ clearance across the blood-brain barrier in Alzheimer’s disease

                         P21 Reiner F. Haseloff (Leibniz-Institute of Molecular Pharmacology, Berlin, Germany)
                         Identification of potential biomarkers for evaluation of neurodegenerative
                         disorders – perspectives and pitfalls

                         P22 Hans-Christian Bauer (Paracelsus Medical University, Salzburg, Austria)
                         Protein transport mechanism at the developing mouse choroid
                         plexus: a molecular characterization

                         P23 Aleksandra Szczepkowska (Institute of Animal Reproduction and
                         Food Research, PAS, Olstyn, Poland)
                         Melatonin implantation during long days up-regulates mRNA expression
                         of aquaporins (AQP1 and AQP4) and claudin-2 in the ovine choroid plexus

                         P24 Janina Skipor (Institute of Animal Reproduction and Food Research,
                         PAS, Olstyn, Poland)
                         oes melatonin affect mRNA expression of toll-like receptors in the ovine choroid plexus?

                         P25 Ádám Nyúl-Tóth (Biological Research Centre, HAS, Szeged, Hungary)
                         Toll-like receptors in brain endothelial cells: expression and role in barrier regulation

                         P26 Imola Wilhelm (Biological Research Centre, HAS, Szeged, Hungary)
                         Role of PACAP in the regulation of survival and barrier properties of cerebral
                         endothelial cells

19:00               Wine tasting and dinner